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DEPARTMENT OF LABORATORY SERVICES

Our Laboratory consists of four core divisions and each division is headed by a consultant in that specialty with a team of medical and paramedical staff. Our team is committed to excellence in the field of medical laboratory services and strives to provide a highest quality comprehensive diagnostic laboratory facilities to patients and clinicians. The laboratory provides service 24 hours a day, seven days a week to meet the clinical needs of our inpatients and outpatients.

Core Divisions

  1. Chemical Pathology Lab
  2. Hematology Lab
  3. Histopathology Lab
  4. Microbiology Lab
  5. Clinical Virology Lab

 


CHEMICAL PATHOLOGY LAB

Overview

Bio chemical analysis of blood and body fluids is done at Chemical pathology laboratory. All routine clinical biochemistry tests, special chemistry tests and immunoassay tests are performed with fully automated bio chemistry and immunoassay analyzers.

  • Routine chemistry tests are performed daily and reports are available to collect by the relevant wards on the same day. Urgent test results and critical values are informed to the relevant wards over the phone immediately after result validation.
  • Lipid profile test is performed on Mondays and Thursdays and dates are given to clinic patients to ensure the reports are available for next clinic visit.
  • Immunoassays including hormone (thyroid profile, LH, FSH, Testosterone, cortisol, HCG), tumor markers (CA 125, CEA, PSA, Alpha feto protein), troponin I and ferritin are performed at chemical pathology laboratory.
  • Separate laboratory sections are available in Dialysis unit and OPD.
  • In dialysis unit laboratory routine biochemistry tests of patients of renal unit and clinic are performed. Apart from that certain immunoassay tests are performed in immunoassay analyzer available at dialysis unit laboratory. Facilities to perform Parathyroid hormone, Vitamin D level, vitamin B 12 levels are available.
  • Urine full report analysis is performed in OPD laboratory.
  • Night laboratory
  • Facilities to do routine bio chemistry tests are available at night laboratory. Troponin I test is performed in night laboratory using point of care testing analyzer).
  • Accuracy of test results is ensured with daily internal quality control process and participating in internationally recognized external quality assurance programs.
  • Consultant Chemical pathologist will provide opinion on line of investigations for patients with metabolic disorders, electrolyte disturbances and on analytical aspect of abnormal results.
  • Advice on patient preparation, sample collection and process of send away samples to other laboratories will be provided by chemical pathology laboratory

 

1. ROUTINE INVESTIGATIONS

Accepting from 8.00am to 2.30 pm –week days /Sundays/ PHs and 8.00 am to 10.30 am on Saturdays

Serum investigations

Tube

Special remarks

AST/SGOT

Plain tube

 

ALT/SGPT

Plain tube

 

ALP

Plain tube

 

Total Protein

Plain tube

 

Albumin

Plain tube

 

Globulin

Plain tube

 

Gamma GT

Plain tube

 

Bilirubin (Total /Direct)

Plain tube

 

Amylase

Plain tube

 

Creatinine

Plain tube

 

CK

Plain tube

 

Cholesterol (Total)

Plain tube

 

CRP

Plain tube

 

Total Calcium/corrected calcium

Plain tube

 

Ionize Calcium

Plain tube

 

Electrolytes(NA+,K+,Cl-)

Plain tube

 

Iron studies (Fe,TIBC ,% saturation)

Plain tube

 

Lithium

Plain tube

 

Magnesium

Plain tube

 

Phosphorus

Plain tube

 

Uric acid

Plain tube

 

Urea

Plain tube

 

HBA1C

EDTA tube

Need consultant signature and stamp

FBS

Sugar tube

 

PPBS

Sugar tube

Sample should be received to lab before 2.30 pm

 

2. URINE INVESTIGATIONS

Urine investigations

Container

Special remarks

Electrolytes(Na+/K+/Cl-)

Urine Bottle

 

Calcium

Acid washed tube

 

Phosphorus

Urine Bottle

 

Creatinine

Urine Bottle

 

Protein

Urine Bottle

 

Protein /Creatinine ratio (spot sample)

Urine Bottle

1st /2nd morning urine sample

Calcium/ Creatinine ratio

Urine Bottle

 

Potassium /Creatinine ratio

Urine Bottle

 

Uric acid

Urine Bottle

 

Amylase

Urine Bottle

 

 

Urine for Bence jones protein

(screening test)

Urine Bottle

 

 

Urine full report

 

Urine Bottle

 

Test done at OPD lab (Room No 15)

Urine Strip Test

(pH, specific gravity, Ketone bodies, Bilirubin, Urobilinogen, protein)

Urine bottle

Test done at OPD lab (room No 15)

 

  1. CSF for Sugar and Protein -Special tube provided by chief MLT room (Room no 5)
  2. Biochemical analysis of
    • Pleural Fluid
    • Peritoneal Fluid

 


 

SPECIAL INVESTIGATIONS

1.DATE NEED TO BE TAKEN FROM BOICHEMISTRY LAB

Investigations

Container

Special remarks

24 hrs urine creatinine clearance

Special bottle provided by lab

 

24 hrs urine Protein Excretion

Special bottle provided by lab

 

24 hrs urine Calcium Excretion

Special bottle provided by lab

 

24 hrs urine Phosphate Excretion

Special bottle provided by lab

 

24 hrs urine Sodium and Potassium Excretion

Special bottle provided by lab

 

24 hrs urine Uric acid Excretion

Special bottle provided by lab

 

24 hrs urine Magnesium Excretion

Special bottle provided by lab

 

24 hrs urine For VMA

Special bottle provided by lab

 

24 hrs urine For Metanephrine

Special bottle provided by lab

 

OGTT

Sugar tube

 

Lipid Profile

Plain tube

Need consultant signature and stamp

Cryoglobulin

 

Need consultant signature and stamp

With proper history of the patient, instructions sampling done at biochemistry lab

 

2.IMMUNO ASSAY TESTS -HORMONE TESTS ,TESTS FOR TUMOR MARKERS AND CARDIAC MARKERS (need proper request form with patient history consultant signature and stamp)

Samples accepting from 8.00am to 2.30 pm –week days / PHs and 8.00 am to 10.30 am on Saturdays .

Investigation

Tube

Special remarks

TSH/FT4/FT3

Plain tube

 

FSH

Plain tube

 

LH

Plain tube

 

Cortisol

Plain tube

Overnight fasting

9am Prolactin

Plain tube

Overnight fasting

9am Testosterone

Plain tube

Overnight fasting

PSA

Plain tube

 

CEA

Plain tube

 

AFP

Plain tube

 

CA125

Plain tube

 

Beta HCG

Plain tube

 

Serum Ferritin

Plain tube

 

Troponin I

Plain tube

Accepting as a routine test

 

3. INVESTIGATIONS DONE AT DIALYSIS UNIT

9.00 am PTH

Plain tube

Overnight fasting, Date need to be taken from biochemistry lab, Sampling done at DU lab.

9 .00am ACTH

Iced EDTA tube

Overnight fasting ,Date need to be taken from biochemistry lab, Sampling done at DU lab

Vit D level

Plain tube

Overnight fasting ,Date need to be taken from biochemistry lab, Sampling done at DU lab

Vit B12 level

Plain tube

Overnight fasting ,Date need to be taken from biochemistry lab, Sampling done at DU lab

Serum Osmolality

Plain tube

 

Urine Osmolality

Urine bottle

 

Tacrolimus level

Plain tube

 

Cyclosporine level

Plain tube

 

Gentamycin level

Plain tube

 

Vancomycin level

Plain tube

 

 

4. SEND AWAY INVESTIGATIONS

24 hrs urine For VMA

Special bottle provided by biochemistry lab

Date need to be taken from biochemistry lab, sample accepting to biochemistry lab and sending to MRI for investigation.

24 hrs urine For Metanephrine

Special bottle provided by biochemistry lab

Date need to be taken from biochemistry lab ,sample accepting to biochemistry lab and sending to MRI for investigation

Renin / Aldosterone

 

Need consultant referral

 

 

Performance

 

Staff

Chemical Pathology Lab

Investigation 2017 2016 2015
TSH 19631 16819 15091
T4 9660 8956 9346
FT3 3360 3114 3270
Prolactin 1068 1020 729
LH 783 718 373
Troponin I 9797 8442 5032
CA 125 1508 1338 1321
PSA 1593 730 690
FSH 784 690 637

 

 

Room No. 17

 

 Consultant Chemical Pathologist
 Dr. (Mrs.) R. D. P. Jayawardana
 MBBS, D(Path), MD(Chem. Path.)

Biochemistry Lab

Medical Officers 2
Registrar 1
MLT 15
Lab Orderly 4
SKS 4
   

OPD Lab

 
MLT 5
Lab Orderly 2
SKS 2
   

OPD Lab

 
MLT 7
Lab Orderly 2
SKS 2


Milestones / Special Events

   
 

Future Goals

   
  • Capacity building of staff
  • Outreach services
   

 


HEMATOLOGY LAB

Overview

The department of Haematology provides comprehensive range of laboratory and clinical services. It serves the patients of teaching hospital Kandy as well as the patients from the surrounding hospitals and adjacent provinces. Haematology laboratory also provides services for the National Thalassaemia Screening program of the Central Province

Laboratory Services

Haematology laboratory provides services for the National Thalassaemia Screening program for the central province. The service provides by the laboratory covers a wide range of tests which are performed with fully automated equipment.

The laboratory is capable of processing routine hematological investigations such as FBC, PT & INR, APTT, and ESR with high degree of accuracy and within minimum turnaround time.
In addition, it offers the diagnosis of coagulation disorders, anaemia, haemoglobinopathies, haemolytic anaemia and thrombophilia.

The laboratory is also equipped with a new generation flow cytometry machine which enables the diagnosis of acute/chronic leukaemia, lymphoma. In order to maintain high standards of analysis our hematology department regularly participates internal and national external quality assurance programme.

Clinical Services

Patients from the outpatient department, clinics, and ward of teaching hospital Kandy and other outside hospitals are referred to our clinic for specialized haematological assessments and management.

The clinics run from Monday to Friday in the morning session which are conducted by two consultant haematologists.
The ward consist of 20 beds with trained staff which particularly focus on management of congenital bleeding discords such as haemophilia, thrombotic events, bone marrow failure syndrome and haematological malignancy.

Management of clinics and inward patients are under the preview of two consultant haematologists separately as unit A and B.
Further, a development program me is already underway for the improvement of the haematology department of Teaching hospital Kandy to provide an enhanced quality service to a larger segment of patients.

Establishing Haematology Unit B was started in June 2016 and is still under way..

Performance

 

Staff

Hematology Lab

Investigations    
Year 2017 2016 2015
FBC 150997 147997 56301
Hb 2471 1601 4873
Blood picture 21972 22256 22568
Retic count 1258 1397 1232
PCV 35 27 49
ESR 22173 22523 11651
PT 57259 64673 45561
APTT 8863 7259 7435
TT 7 19 43
Fibrinogen assay 76 24 18
D dimer 6 2 -
Bleeding time 3113 3979 5689
       
Special Coagulation Tests      
Mixing study with normal plasma 17 20 26
Mixing study with factor vii deficient plasma 4 6 5
Mixing study with factor viii deficient plasma 4 6 5
Mixing study with factor ix deficient plasma 4 6 5
Mixing study with factor x deficient plasma 4 6 5
Mixing study with factor xi deficient plasma 4 6 6
Mixing study with factor xii deficient plasma 4 6 6
Factor assay 4 10  
Inhibitory Screening 04 12 09
Urea clot solubility test 14 12 07
       
Hemolytic Investigations      
Osmotic fragility test 18 16 20
G6PDScreening test 141 150 170
Kleihauer test 1 3 2
Heinz bodies 4 5 6
Hb H inclusions 7 5 15
HAMS test 99 85 75
HPLC/Capill.Hb Electrophoresis 1008 861 1037
Urine haemosiderine test 112 100 76
Donath Landsteiner test 7 01 01
Flowcytometry analysis      
Acute leukaemia panel 20 - -
Chronic lymphoproliferative panel 10 - -
Myeloma Panel 1 - -
Thromboelastometry(ROTEM) 61 58 125
Urine Test (Haemosiderin/ Hb) 112 100 76
Methhaemoglobin 1 4 1
Bone marrow biopsy A side 493 647 690
Bone marrow biopsy B side 228 90 -
       
Thrombophilia Investigations      
Antithrombin 111 97 10 -
Protein C Assay 110 25 10
Protein S assay 112 21 10
Lupus anticoagulant 212 27 44
Factor v leiden mutation 6 - -
HBA1c 1797 3685 2915
S.Protein electrophoresis 949 785 500
Serum protein immunophenotyping 59 84 -
Urine for spectroscopy 8 13 8

 

Haematology Clinic A

Inward Patient Care - Ward 52

Year 2017 2016 2015 2014
Beds 10 10 20 20
Admissions 1387 1151 1011 781
Bed occupancy ratio 124% 115% 85% 76%
Average length of stay        

 

Outpatient Care

Location – Second Floor of OPD building (Room No.22)
Extension : 2251

Year 2017 2016 2015 2014
Clinic Visits (Total) 7227 7838 8342 8369
First Clinic Visit 569 5911 586 590
Average number of patient per clinic 50 54 58 58

 

 


 

Haematology Clinic B

Haematology clinic (B) was established in room 12 of the ground floor of medical complex building in July 2018.
The clinic started fully functioning with a permanent nursing officer from January 2019.

Inward Patient Care - Ward 52

Year 2017 2016 2015 2014
Beds 10 10    
Admissions 148 28    
Bed occupancy ratio 80% 70%    
Average length of stay        

 

Investigations done at Hematology lab

Year Test Tube Special Preparations and Instructions
General Haematology ESR Citrated (1.6:0.4)  
Full blood count EDTA (2ml/1ml/750µl)  
Reticulocyte count EDTA  
Blood picture EDTA Collect sample before blood transfusion
PT/INR Sodium citrate (1.8:0.2) Collect up to the mark and mix gently to avoid clot formation
APTT Sodium citrate  

 

Histopathology Lab

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Special Haematology

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Special Haematology

Category

Test

Tube

Special preparations and Instructions

 

Investigations for bleeders

Thrombin time

Sodium citrate

 

Fibrinogen level

Sodium citrate

 

D.Dimer

Sodium citrate

 

Clot solubility test

Sodium citrate

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Consultant referral required

Factor correction

Sodium citrate

Mixing studies with specific factor deficient plasma

Sodium citrate

Inhibitory screening

Sodium citrate

Thromboelastometry

(ROTEM)

Sodium citrate

 

 

Thrombophilia

investigations

Protein C level

Sodium citrate

Stop vitamin K antagonists 2 weeks before

testing

 

Protein S level

Sodium citrate

Antithrombin III level

Sodium citrate

Activated protein C resistance

Sodium citrate

Lupus anticoagulant

Sodium citrate

Haemoglobinopathy

Capillary Hb electrophoresis

EDTA

Collect before blood transfusion

HbH inclusion

EDTA

 

 

 

Haemolytic anaemia

investigatons

 

G6PD deficiency screening (Brewer’s test)

EDTA

Collect before blood transfusion

Osmotic fragility test

Heparinized tube/EDTA

 

 

 

 

 

 

 

 

Consultant referral required

HAM test

EDTA

Collect before blood transfusion

Group AB or ABO compatible blood as control

Heinz body test

EDTA

 

Methaemoglobin level

EDTA

 

Donath Landsteiner test

Plain khan tube

 

Other blood tests

Kleihauer test

 

EDTA

 

Bone marrow biopsy

Bone marrow aspiration

EDTA

 

Trephine biopsy

 

Picric acid

 

Flowcytometry(peripheral blood /bone marrow aspirate)

EDTA

 

Urine tests

Urine for Haemoglobin

 

Plain tube

   

Urine for Haemosiderin

Acid washed plain tube

Collect first morning specimen for 3 consecutive days

 

Weekdays -Samples are accepted from 8.00am to 3.00 pm
Saturday from 8am to 11 am
Sunday & PH 8.00 am to 2.00pm

 

Hematology Lab

Extension : 2278
Location Top Floor of OPD building

Consultants

 Unit A:
 Dr. (Mrs.) B. M. S. B. Athauda
 MBBS, D.Path., MD

 

 Unit B:
 Dr. (Mrs.) V. C. P. Gunawardana
 MBBS, MD, MRCP/Haem Diploma UK
 ISO assessor in Haematology/SLAB

PG Trainees 7 Both units
Medical Officers Unit A: 04
Unit B: 04
MLTs 12


Milestones / Special Events

   
  • Flow cytometry machine was installed in 2017

Future Goals

   

 

  • Establishment of cytogenetics& Molecular Lab.
   

 


 

HISTOPATHOLOGY LAB

Overview

Histopathology and cytopathology investigations are carried out in the Department of Histopathology.
In addition, we provide specilised ancillary services which are not available in most of the other hospitals in Sri Lanka which include immunohistochemistry and immunofluorescence service.

  • Histology laboratory performed routine surgical and cytology specimen processing which is done all seven days of the week.
  • Histology Service: All the surgical and endoscopic samples are sent to the histology laboratory every day from operating theatres and other units..

 

Performance

 

Staff

Histology of surgical samples

Test Preparation How to send to the lab When report is ready Where to collect the report

Histology of surgical samples

-Specimen should be sent with a properly filled yellow colour request form.

 

-Please mention reference numbers of previous histology, FNA and cytology reports and IHC studies if available.

 

-Booklets should be collected from the Histopath lab – Room No 4.

-All the samples should be sent to the Histopath lab – Room No 4 within 4 hours of surgery.

(If there is undue delay please inform the Registrar in Histopathology (Ext: 2226)

 

-Should use an adequate container.

 

-Should add 10% buffered formal saline – 2 to 10 times of volume of the sample.

Report will be available within 3-7 days of surgery unless special procedure is needed.

Reports should be collected from the Histopath lab – Room No 4

 

Fine needle aspiration

 

Test Preparation How to send to the lab When report is ready Where to collect the report

Fine needle aspiration

-Patient should be sent to the Histopath lab – Room No 2 with a properly filled yellow colour request form.

No prior appointment is necessary.

FNAC is performed from Monday to Friday 8am to 4pm and Saturday 8am to 12pm

PH/ Sunday- No service is available

 

-Please mention reference numbers of previous tests.

 

-Booklets should be collected from the Histopath lab – Room

-Surgical gloves, 3 23G needles and a 10cc needle should be sent to the lab when the patient is sent from wards.

Report will be available within 1-2 days of procedure.

Same day report is available on request

Reports should be collected by wards and clinics, from the Histopath lab – Room No 4

 

Cytology of fluid samples

 

Test Preparation How to send to the lab When report is ready Where to collect the report

Cytology of fluid samples

 

-Specimen should be sent with a properly filled yellow colour request form.

-Booklets should be collected from the Histopath lab – Room No 4

-Please mention whether biopsy taken or not and previous reference numbers.

 

-Fresh samples should be sent to the Histopath lab - Room No 4 as soon as possible (within 2hrs).

 

-If any delay, store at 40C (in the door of refrigerator).

-Smears prepared on glass slides should be fixed in 95% alcohol for 20 minutes immediately after smearing.

Report will be available within 1-2 days of procedure.

Same day report is available on request

Reports should be collected by wards and clinics, from the Histopath lab – Room No 4

 

 Cervical cytology smears

 

Test Preparation How to send to the lab When report is ready Where to collect the report

Cervical cytology smears

 

 

-Smears should be sent to the Histopath lab – Room No 4 with a properly filled request form.

 

-Please mention previous reference numbers.

 

-Booklets should be collected from the Histo lab – Room No 4

-Smears should be fixed

In 95% alcohol for 20 minutes.

Report will be available within 3-4 days of procedure.

Same day report is available on request

Reports should be collected by wards and clinics, from the Histopath lab – Room No 4

 

Frozen sections

 

Test Preparation How to send to the lab When report is ready Where to collect the report

Frozen sections

 

 

-It should be discussed with the Histopath lab one day prior to the surgery and should be informed one hour before the surgery.

 

-Samples should be sent to the Histopath lab – Room No 4 with a properly filled yellow colour request form.

 

-Please mention the test as fresh frozen sections at the top of the request form and previous reference numbers if available.

 

-Booklets should be collected from the Histo lab – Room No 4

-Fresh samples should be kept over a gauze soaked with normal saline and sent to the Histopath lab – Room No 4, within 10 minutes.

 

Report will be available within 20-30 minutes of procedure.

-Findings will be informed over the phone.

 

-Reports should be collected by wards and clinics, from the Histopath lab – Room No 4

 

Immunohistochemistry

Test Preparation How to send to the lab When report is ready Where to collect the report

Immunohisto-

Chemistry

 

-Histology done at Teaching Hospital IHC will be decided by the reporting consultant.

Currently

If IHC service is required from other hospitals: Wax blocks should be sent with a properly filled request form

(IHC Request forms can be collected from the histology lab).

 

 

-Please mention reference numbers of previous histology, FNA and cytology reports and IHC studies if available.

 

-Booklets should be collected from the Histopath lab – Room No 4.

-Wax block with H & E slide should be sent with a copy of the histology report to the Histopath lab – Room No 2.

 

 

Report will be available within a week of histology report unless special procedure is needed.

Reports should be collected from the Histopath lab –Room No 4

 

Contact extension 2226 for any inquiry

 

Immunofluorescence

Test Preparation How to send to the lab When report is ready Where to collect the report

Test

Preparation

How to send to the lab

When report is ready

Where to collect the report

Immuno-

fluorescence

 

-It should be discussed with the Histopath lab one day prior to the procedure and should be informed one hour before the procedure.

 

-Samples should be sent to the Histopath lab – Room No 4 with a properly filled yellow colour request form.

 

-Please mention the test as immuno-fluoroscence at the top of the request form and previous reference numbers if available.

 

-Please mention reference numbers of previous histology, FNA and cytology reports and IHC studies if available.

 

-Booklets should be collected from the Histopath lab – Room No 4.

-Fresh samples should be kept over a gauze soaked with normal saline and sent to the Histopath lab – Room No 4, within 10 minutes.

 

 

 

Report will be available within 20-30 minutes of procedure.

-Findings will be informed over the phone.

 

-Reports should be collected from the Histopath lab –Room No 4

 

 Special stains

Test Preparation How to send to the lab When report is ready Where to collect the report

Special stains

Perl stain for iron

PAS+D

Alcian blue

AFB

Fite stain

Giemsa stain

Reticulin

Grocotte

Copper stain

Colloid Iron

 

 

-For TH Kandy samples – necessary special stains will be decided by the consultant pathologist.

 

Other hospitals: Specimen should be sent with a properly filled yellow colour request form.

 

-Please mention reference numbers of previous histology, FNA and cytology reports and IHC studies if available.

 

-Booklets should be collected from the Histopath lab – Room No 4.

-All the samples should be sent to the Histopath lab – Room No 4 within 4 hours of surgery.

 

-Should use an adequate container.

 

-Should add 10% buffered formal saline – 2 to 10 times of volume of the sample.

 

-A fresh sample is needed for special stains for adipocytes (fat cells).

 

-Patient should be sent to the Histopath lab – Room No 2, for stains for amyloid in abdominal fat aspirate.

 

Report will be available within 3-7 days of surgery unless special procedure is needed.

Reports should be collected from the Histopath lab – Room No 4

 

Cell block preparation

Test Preparation How to send to the lab When report is ready Where to collect the report

Cell block preparation

 

-Specimen should be sent with a properly filled yellow colour request form.

 

-Booklets should be collected from the Histo lab – Room No 4

 

 

-At least 2ml of fresh fluid sample with or without particles should be sent to the Histopath lab - Room No 4 as soon as possible.

 

-If any delay, store at 40C (in the door of refrigerator).

 

-Smears should be fixed in 95% alcohol for 20 minutes.

 

Report will be available within 3-4 days of procedure.

Reports should be collected by wards and clinics, from the Histo lab – Room No 4.

 

Examination of Barr body

Test Preparation How to send to the lab When report is ready Where to collect the report

Examination of Barr body

 

-Please discuss with the Histopath lab (2226) one-hour prior.

 

-Patient should be sent with a properly filled yellow colour request form.

 

-Booklets should be collected from the Histo lab – Room No 4

 

 

-Patient should be sent to the Histopath lab – Room No 2 with a parous woman.

Report will be available within 1 hour of procedure.

Reports should be collected by wards and clinics, from the Histopath lab – Room No 4.

 

Slit skin smears

Test Preparation How to send to the lab When report is ready Where to collect the report

Slit skin smears

 

-Specimen should be sent with a properly filled yellow colour request form.

 

-Booklets should be collected from the Histopath lab – Room No 4

 

 

 

-Air dried smears should be sent to the Histopath lab – Room No 4.

Report will be available within 3-4 days of procedure.

Reports should be collected by wards and clinics, from the Histopath lab – Room No 4.

 

 Crush/ squash smears

Test Preparation How to send to the lab When report is ready Where to collect the report

Crush/ squash smears

 

 

 

-It should be discussed with the Histopath lab one day prior to the surgery and should be informed one hour before the surgery.

 

-Samples should be sent to the Histopath lab – Room No 4 with a properly filled yellow colour request form.

 

-Please mention the test as fresh frozen sections at the top of the request form and previous reference numbers if available.

 

-Booklets should be collected from the Histo lab – Room No 4

-Fresh samples should be kept over a gauze soaked with normal saline and sent to the Histopath lab – Room No 4, within 10 minutes.

 

Report will be available within 20-30 minutes of procedure.

-Findings will be informed over the phone.

 

-Reports should be collected by wards and clinics, from the Histopath lab – Room No 4

 

Post Mortems:

Pathological post mortems can be performed on request.

This is usually asked for by the patient’s doctor, either to provide information about the illness or cause of death, or to advance medical research. Hospital post-mortems can only be carried out with consent from the next of kin.

Hospital post-mortems are important in helping to understand how people die. For example, some illnesses are passed down through families and a post-mortem examination may provide you with this information if you wish to know. A post-mortem can also help doctors to understand illnesses and may also contribute to the development of new ways of treating patients with similar problems. Hospital post-mortems can be limited to certain areas of the body, such as the head, chest or abdomen, and this will be discussed with you when you are asked for your consent. Only the specific organs or tissue which you give your consent can be removed for examination.

Consent of the next of kin should be obtained followed by the authority from the hospital administration. The written request should be sent to the consultant pathologist and the time will be arranged with consultation of the pathologist and the team.

Death certificate should be issued prior to the post mortem and the post mortem is performed mainly for academic purposes.

The medical officer of the ward should be present at the post mortem to identify the body.

 

  • Referrals and second opinion: Wax blocks, slides, clinical photographs should be sent to the relevant consultant with a referral letter.

 

Performance

Investigation 2018 (till Nov.) 2017 2016
Histology 11500 12500 11560
Cytology 3000 2500 2450
Fine needle aspiration 2600 3012 2600
Cervical Cytology 3500 3800 3600
Immunohistochemistry 1500 550 250
Post Mortem 250 200 234

 

 

Histopathology Lab

Extension : 2278
Location Top Floor of OPD building – room number 04

 Dr. R. M. P. Rathnayaka
 Consultant Pathologist, DPath MD

 

 Dr. (Mrs.) A. Vithanage
 Consultant Pathologist, DPath MD

Medical Officers Grade medical officers - 04
Post graduate trainees of different levels - 12
MLTs 08
Lab orderly 02


Milestones / Special Events

   
  • Automated Immunohistochemistry machine was installed - 2017
  • Immmunofluorescence services started - 2018
  • Frozen sections per operative service - 2018

Future Goals

   

 

  • Establishment of genetic lab to analyze common genetic aberrations in malignancies.
  • Establishment of electron microscopy services.
   

 


 

MICROBIOLOGY LAB

Overview

The Lab offers 24 hours service performing diagnostic Bacteriology and Mycology investigations from the samples received from both in-patients and clinics in Teaching Hospital Kandy and also from several outreach health centers in central province.

 


 

The Bacteriology Section

  • Isolates and identifies clinically significant microorganisms from clinical specimens such as blood, sputum, cerebrospinal fluid, other body fluids and urine and performs antimicrobial susceptibility testing on these bacterial pathogens. These functions are performed with the Vitek-2 automated instrument.
  • Additional reference identification and susceptibility testing methods for other, more fastidious bacterial agents are also available.
  • Performs blood cultures using the Bactec system, which provides continuous monitoring of blood cultures for the entire 5-day incubation period.

The Mycology Section

  • Performs identification and anti-fungal susceptibility testing on clinically significant yeast isolates.
  • Provides identification of pathogenic moulds recovered from clinical specimens, including dermatophytes, moulds causing wound and systemic infections, and systemic mycotic agents such as Histoplamsa capsulatum and Blastomyces dermatiditis.

Others

  • Performs Procalcitonin using Mini-vidas for selected patients with sepsis.

 

Performance

 

Staff

Microbiology Lab

Year  2017  2016  2015
Urine  Cultures  24355  24155  21560
Sputum Cultures  4194  3192  3384
Blood Cultures  18927  19511  20116
CSF/Fluid Cultures  3173  3046  3337
Pus Cultures  9829  11056  9130
Direct Smear  47525  42950  30415
CSF/Fluid Full Report  3293  2922  3454
Leprosy  74  54  60
Fungal Cultures  3224  2754  3293
MRSA  5346  5126  5054

 

 

Microbiology Lab

Extension: 2275
Location Room No. 13

 Consultant
 Dr. M. Kotalawala
 MBBS, Dip(Micro), MD(MED-Micro),MPH

Medical Officers  04
MLTs  09
Lab Orderly  06


Milestones / Special Events

   
 

Future Goals

   

 

   

 

 

 


 

CLINICAL VIROLOGY LAB

Overview

The Department of Virology is offering both clinical and diagnostic virology services to TH Kandy & other hospitals in central part of the country. Laboratory is equipped with modern molecular and serological diagnostic facilities and provides testing for a wide variety of viral infections. Established in 2015 with the concept of expanding clinical virology services in Sri Lanka, the Ministry of Health has identified and upgraded the laboratory as a Regional Clinical Virology Laboratory. In addition to clinical services, the laboratory is functioning as a Sub National Laboratory for Human Influenza Virus Surveillance. The vision of the laboratory is to provide timely and accurate test results enabling optimal patient management.

 

Available Laboratory Tests

  • Molecular Assays
    • Influenza A / B/ H 1 N 1 RNA detection RT-PCR
    • Alpha herpes virus DNA detection PCR assay (HSV/ VZV)
    • CMV DNA quantification PCR
    • BKV DNA quantification PCR
    • EBV DNA quantification PCR
    • Hepatitis B DNA quantification PCR
    • Hepatitis C RNA quantification RT-PCR
    • EBV DNA quantification PCR
    • Dengue virus RNA detection RT-PCR
    • Respiratory virus detection panel (RSV/ Adeno/ Corona/ PIV) – only during outbreaks/ post-transplant
  • Serology Assays
    • Hepatitis Bs antigen
    • Hepatitis Bs antibodies (quantitative)
    • Hepatitis B serology profile (HepB’e’ antigen/ antibody, HepB core total Ab)
    • Hepatitis C antigen/ antibodies
    • Hepatitis A IgM/ IgG
    • CMV IgM/ IgG
    • EBV IgM/ IgG
    • Varicella IgM/ IgG
    • Rubella IgM/ IgG
    • HSV 1& 2, IgM/ IgG
    • Parvo virus B19 IgM
    • Dengue NS1
    • Dengue IgM/ IgG (Flavi virus IgM/IgG)
    • Hantavirus IgM/ IgG
    • Measles IgG
    • Chickungunya IgM


Sample collection and transport

  • Blood for Serology
    Collect 3mL of venous blood in to sterile plain tube & transport in room temperature to the laboratory. If any delay in transport can keep in refrigerator (+04 0 C) for 24 hours. Do not freeze. Haemolized or contaminated samples are not suitable for serology.

 

  • Blood for Molecular diagnosis
    Collect 3mL of venous blood in to EDTA tube & transport in ice as soon as possible to the laboratory. If any delay in transport can keep in refrigerator (+04 0 C) up to 24 hours. Do not freeze. Haemolized or contaminated samples are not suitable for serology.

 

  • Respiratory virus detection
    Collect nasopharyngeal swabs/ aspirates, ET secretions, BAL samples in to viral transport medium (VTM) & transport in ice as soon as possible to the laboratory. If any delay in transport can keep in refrigerator (+04 0 C) up to 24 hours. Do not freeze.

 

  • Tissue samples for virus detection
    Collect small piece of tissue in to sterile, screw capped container & add viral transport medium (VTM) or normal saline/ alcohol. Do not add formaldehyde. Transport in ice as soon as possible to the laboratory. If any delay in transport can keep in refrigerator (+04 0 C) up to 24 hours. Do not freeze.

 

  • Urine samples for CMV/BKV PCR
    Collect clean catch urine (05 mL) in to sterile screw capped disposable container. Transport in ice as soon as possible to the laboratory. If any delay in transport can keep in refrigerator (+040C) up to 24 hours.

 

  • CSF
    Collect in to sterile screw capped disposable container & transport in ice as soon as possible to the laboratory. If any delay in transport can keep in refrigerator (+04 0 C) up to 24 hours. Do not freeze.

Note

  • When you are requesting more than one test, please send a single sample with adequate volume and a single request form.
  • For clarifications, please contact Virology Laboratory, TH Kandy Ext 2491(doctors’ room), 2480 (laboratory).

Performance

 

Staff

Virology Laboratory

Year 2016 2016 2016 2017
         
         
Total        

 

 

Suwapiyasa,
Kappetipola Mawatha, Kandy
Extension 2491/2480

 
   

 Consultant
 Dr. M.A.R.V. Muthugala
 MBBS, PG Dip. Med. Microbiology, MD(Virology)

Medical Officers 02
MLTs 04
Lab Orderly 01


Milestones / Special Events

   
 

Future Goals

   

 

  • Capacity building of staff
  • Outreach services
   

 

 

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