Article Index

 

HISTOPATHOLOGY LAB

Overview

Histopathology and cytopathology investigations are carried out in the Department of Histopathology.
In addition, we provide specilised ancillary services which are not available in most of the other hospitals in Sri Lanka which include immunohistochemistry and immunofluorescence service.

  • Histology laboratory performed routine surgical and cytology specimen processing which is done all seven days of the week.
  • Histology Service: All the surgical and endoscopic samples are sent to the histology laboratory every day from operating theatres and other units..

 

Performance

 

Staff

Histology of surgical samples

Test Preparation How to send to the lab When report is ready Where to collect the report

Histology of surgical samples

-Specimen should be sent with a properly filled yellow colour request form.

 

-Please mention reference numbers of previous histology, FNA and cytology reports and IHC studies if available.

 

-Booklets should be collected from the Histopath lab – Room No 4.

-All the samples should be sent to the Histopath lab – Room No 4 within 4 hours of surgery.

(If there is undue delay please inform the Registrar in Histopathology (Ext: 2226)

 

-Should use an adequate container.

 

-Should add 10% buffered formal saline – 2 to 10 times of volume of the sample.

Report will be available within 3-7 days of surgery unless special procedure is needed.

Reports should be collected from the Histopath lab – Room No 4

 

Fine needle aspiration

 

Test Preparation How to send to the lab When report is ready Where to collect the report

Fine needle aspiration

-Patient should be sent to the Histopath lab – Room No 2 with a properly filled yellow colour request form.

No prior appointment is necessary.

FNAC is performed from Monday to Friday 8am to 4pm and Saturday 8am to 12pm

PH/ Sunday- No service is available

 

-Please mention reference numbers of previous tests.

 

-Booklets should be collected from the Histopath lab – Room

-Surgical gloves, 3 23G needles and a 10cc needle should be sent to the lab when the patient is sent from wards.

Report will be available within 1-2 days of procedure.

Same day report is available on request

Reports should be collected by wards and clinics, from the Histopath lab – Room No 4

 

Cytology of fluid samples

 

Test Preparation How to send to the lab When report is ready Where to collect the report

Cytology of fluid samples

 

-Specimen should be sent with a properly filled yellow colour request form.

-Booklets should be collected from the Histopath lab – Room No 4

-Please mention whether biopsy taken or not and previous reference numbers.

 

-Fresh samples should be sent to the Histopath lab - Room No 4 as soon as possible (within 2hrs).

 

-If any delay, store at 40C (in the door of refrigerator).

-Smears prepared on glass slides should be fixed in 95% alcohol for 20 minutes immediately after smearing.

Report will be available within 1-2 days of procedure.

Same day report is available on request

Reports should be collected by wards and clinics, from the Histopath lab – Room No 4

 

 Cervical cytology smears

 

Test Preparation How to send to the lab When report is ready Where to collect the report

Cervical cytology smears

 

 

-Smears should be sent to the Histopath lab – Room No 4 with a properly filled request form.

 

-Please mention previous reference numbers.

 

-Booklets should be collected from the Histo lab – Room No 4

-Smears should be fixed

In 95% alcohol for 20 minutes.

Report will be available within 3-4 days of procedure.

Same day report is available on request

Reports should be collected by wards and clinics, from the Histopath lab – Room No 4

 

Frozen sections

 

Test Preparation How to send to the lab When report is ready Where to collect the report

Frozen sections

 

 

-It should be discussed with the Histopath lab one day prior to the surgery and should be informed one hour before the surgery.

 

-Samples should be sent to the Histopath lab – Room No 4 with a properly filled yellow colour request form.

 

-Please mention the test as fresh frozen sections at the top of the request form and previous reference numbers if available.

 

-Booklets should be collected from the Histo lab – Room No 4

-Fresh samples should be kept over a gauze soaked with normal saline and sent to the Histopath lab – Room No 4, within 10 minutes.

 

Report will be available within 20-30 minutes of procedure.

-Findings will be informed over the phone.

 

-Reports should be collected by wards and clinics, from the Histopath lab – Room No 4

 

Immunohistochemistry

Test Preparation How to send to the lab When report is ready Where to collect the report

Immunohisto-

Chemistry

 

-Histology done at Teaching Hospital IHC will be decided by the reporting consultant.

Currently

If IHC service is required from other hospitals: Wax blocks should be sent with a properly filled request form

(IHC Request forms can be collected from the histology lab).

 

 

-Please mention reference numbers of previous histology, FNA and cytology reports and IHC studies if available.

 

-Booklets should be collected from the Histopath lab – Room No 4.

-Wax block with H & E slide should be sent with a copy of the histology report to the Histopath lab – Room No 2.

 

 

Report will be available within a week of histology report unless special procedure is needed.

Reports should be collected from the Histopath lab –Room No 4

 

Contact extension 2226 for any inquiry

 

Immunofluorescence

Test Preparation How to send to the lab When report is ready Where to collect the report

Test

Preparation

How to send to the lab

When report is ready

Where to collect the report

Immuno-

fluorescence

 

-It should be discussed with the Histopath lab one day prior to the procedure and should be informed one hour before the procedure.

 

-Samples should be sent to the Histopath lab – Room No 4 with a properly filled yellow colour request form.

 

-Please mention the test as immuno-fluoroscence at the top of the request form and previous reference numbers if available.

 

-Please mention reference numbers of previous histology, FNA and cytology reports and IHC studies if available.

 

-Booklets should be collected from the Histopath lab – Room No 4.

-Fresh samples should be kept over a gauze soaked with normal saline and sent to the Histopath lab – Room No 4, within 10 minutes.

 

 

 

Report will be available within 20-30 minutes of procedure.

-Findings will be informed over the phone.

 

-Reports should be collected from the Histopath lab –Room No 4

 

 Special stains

Test Preparation How to send to the lab When report is ready Where to collect the report

Special stains

Perl stain for iron

PAS+D

Alcian blue

AFB

Fite stain

Giemsa stain

Reticulin

Grocotte

Copper stain

Colloid Iron

 

 

-For TH Kandy samples – necessary special stains will be decided by the consultant pathologist.

 

Other hospitals: Specimen should be sent with a properly filled yellow colour request form.

 

-Please mention reference numbers of previous histology, FNA and cytology reports and IHC studies if available.

 

-Booklets should be collected from the Histopath lab – Room No 4.

-All the samples should be sent to the Histopath lab – Room No 4 within 4 hours of surgery.

 

-Should use an adequate container.

 

-Should add 10% buffered formal saline – 2 to 10 times of volume of the sample.

 

-A fresh sample is needed for special stains for adipocytes (fat cells).

 

-Patient should be sent to the Histopath lab – Room No 2, for stains for amyloid in abdominal fat aspirate.

 

Report will be available within 3-7 days of surgery unless special procedure is needed.

Reports should be collected from the Histopath lab – Room No 4

 

Cell block preparation

Test Preparation How to send to the lab When report is ready Where to collect the report

Cell block preparation

 

-Specimen should be sent with a properly filled yellow colour request form.

 

-Booklets should be collected from the Histo lab – Room No 4

 

 

-At least 2ml of fresh fluid sample with or without particles should be sent to the Histopath lab - Room No 4 as soon as possible.

 

-If any delay, store at 40C (in the door of refrigerator).

 

-Smears should be fixed in 95% alcohol for 20 minutes.

 

Report will be available within 3-4 days of procedure.

Reports should be collected by wards and clinics, from the Histo lab – Room No 4.

 

Examination of Barr body

Test Preparation How to send to the lab When report is ready Where to collect the report

Examination of Barr body

 

-Please discuss with the Histopath lab (2226) one-hour prior.

 

-Patient should be sent with a properly filled yellow colour request form.

 

-Booklets should be collected from the Histo lab – Room No 4

 

 

-Patient should be sent to the Histopath lab – Room No 2 with a parous woman.

Report will be available within 1 hour of procedure.

Reports should be collected by wards and clinics, from the Histopath lab – Room No 4.

 

Slit skin smears

Test Preparation How to send to the lab When report is ready Where to collect the report

Slit skin smears

 

-Specimen should be sent with a properly filled yellow colour request form.

 

-Booklets should be collected from the Histopath lab – Room No 4

 

 

 

-Air dried smears should be sent to the Histopath lab – Room No 4.

Report will be available within 3-4 days of procedure.

Reports should be collected by wards and clinics, from the Histopath lab – Room No 4.

 

 Crush/ squash smears

Test Preparation How to send to the lab When report is ready Where to collect the report

Crush/ squash smears

 

 

 

-It should be discussed with the Histopath lab one day prior to the surgery and should be informed one hour before the surgery.

 

-Samples should be sent to the Histopath lab – Room No 4 with a properly filled yellow colour request form.

 

-Please mention the test as fresh frozen sections at the top of the request form and previous reference numbers if available.

 

-Booklets should be collected from the Histo lab – Room No 4

-Fresh samples should be kept over a gauze soaked with normal saline and sent to the Histopath lab – Room No 4, within 10 minutes.

 

Report will be available within 20-30 minutes of procedure.

-Findings will be informed over the phone.

 

-Reports should be collected by wards and clinics, from the Histopath lab – Room No 4

 

Post Mortems:

Pathological post mortems can be performed on request.

This is usually asked for by the patient’s doctor, either to provide information about the illness or cause of death, or to advance medical research. Hospital post-mortems can only be carried out with consent from the next of kin.

Hospital post-mortems are important in helping to understand how people die. For example, some illnesses are passed down through families and a post-mortem examination may provide you with this information if you wish to know. A post-mortem can also help doctors to understand illnesses and may also contribute to the development of new ways of treating patients with similar problems. Hospital post-mortems can be limited to certain areas of the body, such as the head, chest or abdomen, and this will be discussed with you when you are asked for your consent. Only the specific organs or tissue which you give your consent can be removed for examination.

Consent of the next of kin should be obtained followed by the authority from the hospital administration. The written request should be sent to the consultant pathologist and the time will be arranged with consultation of the pathologist and the team.

Death certificate should be issued prior to the post mortem and the post mortem is performed mainly for academic purposes.

The medical officer of the ward should be present at the post mortem to identify the body.

 

  • Referrals and second opinion: Wax blocks, slides, clinical photographs should be sent to the relevant consultant with a referral letter.

 

Performance

Investigation 2018 (till Nov.) 2017 2016
Histology 11500 12500 11560
Cytology 3000 2500 2450
Fine needle aspiration 2600 3012 2600
Cervical Cytology 3500 3800 3600
Immunohistochemistry 1500 550 250
Post Mortem 250 200 234

 

 

Histopathology Lab

Extension : 2278
Location Top Floor of OPD building – room number 04

Dr. Palitha Ratnayake
Consultant Pathologist, DPath MD

Dr. (Mrs.) Anoma Vithanage
Consultant Pathologist, DPath MD

Medical Officers Grade medical officers - 04
Post graduate trainees of different levels - 12
MLTs 08
Lab orderly 02


Milestones / Special Events

   
  • Automated Immunohistochemistry machine was installed - 2017
  • Immmunofluorescence services started - 2018
  • Frozen sections per operative service - 2018

Future Goals

   

 

  • Establishment of genetic lab to analyze common genetic aberrations in malignancies.
  • Establishment of electron microscopy services.
   

 

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